there is only one stack per timepoint, this software can be used to stabilize the timeseries (drift-correction). When we speak of multiview datasets we generally mean that per timepoint there n image stacks, which could be different: It has all the functionality the SPIM Registration offered, but is much more flexible and supports many more types of registration, fusion and data handling.Įxamples What does 'multiview' mean exactly? While the SPIM Registration will continue to live within Fiji for the time being, we will mostly offer support only for this new software package. This software package is the successor to the SPIM Registration package. ![]() Both projects share a common XML data model to describe multiview datasets. Interactive viewing and annotation of the data is provided by integration with Tobias Pietzsch's BigDataViewer. The software is designed for lightsheet fluorescence microscopy (LSFM, second box), but is applicable to any form of three or higher dimensional imaging modalities like confocal timeseries or multicolor stacks. The Multiview Reconstruction software package enables users to register, fuse, deconvolve and view multiview microscopy images (first box). Webpageįor technical details about the registration method and SPIM imaging see also SPIM Registration Method. Tomancak (2014) "Efficient Bayesian-based Multiview Deconvolution", Nature Methods, 11(6):645-648. Tomancak (2010) "Software for bead-based registration of selective plane illumination microscopy data", Nature Methods, 7(6):418-419. If you use it successfully for your research please be so kind to cite our work: Please note that the SPIM registration plugin available through Fiji, is based on a publication.
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